| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lucca, Sarah (11)
|
MRHAA |
27.65 |
27.42 |
-0.23 |
| 2 |
Lucca, Julia (11)
|
MRHAA |
28.97 |
28.01 |
-0.96 |
| 3 |
Bak, Julia (11)
|
MRQNS |
29.01 |
28.49 |
-0.52 |
| 4 |
Park, Cadence V (11)
|
MRYFD |
30.11 |
29.42 |
-0.69 |
| 5 |
Birch, Elizabeth K (11)
|
MRAGUA |
29.96 |
29.68 |
-0.28 |
| 6 |
Tedesco, Amanda G (11)
|
MRIA |
30.58 |
30.33 |
-0.25 |
| 7 |
Yang, Chloe (11)
|
MRAGUA |
30.66 |
30.37 |
-0.29 |
| 8 |
Vitiello, Alexis L (11)
|
MRWISC |
30.32 |
30.59 |
0.27 |
| 9 |
Chen, Vivien E (11)
|
MRRA |
30.78 |
30.67 |
-0.11 |
| 10 |
Bendersky, Hailey R (11)
|
MRWISC |
31.20 |
30.70 |
-0.50 |
| 11 |
Guerrero, Mckenzie M (11)
|
MRYMID |
31.22 |
31.08 |
-0.14 |
| 12 |
Wu, Evelyn Y (11)
|
MRYMID |
30.97 |
31.20 |
0.23 |
| 13 |
Spencer-Edwards, Lauren A (11)
|
MRQNS |
30.95 |
31.34 |
0.39 |
| 14 |
Dougherty, Emily W (11)
|
MRBAD |
31.18 |
31.50 |
0.32 |
| 15 |
Behrend, Molly C (11)
|
MRWEST |
30.55 |
31.77 |
1.22 |
| 16 |
Jorsling, Jules A (11)
|
MRBAD |
31.05 |
31.85 |
0.80 |
|
Tamura, Lynn (11)
|
MRWEST |
31.26 |
NS |
- |